WebFeb 12, 2024 · Counter Selection markers used in Clostridium spp. and their mechanisms of selection. The native gene of interest (G.O.I) is represented in red, desired insert in blue, and the counter selection marker gene in dark purple. The green and blue bars represent regions of homology between the chromosome and donor plasmid. WebMay 1, 2024 · The new marker pheS* was constructed for counter-selection in Bacillus amyloliquefaciens.. This marker encodes Bacillus subtilis PheS with two mutations, T255S and A309G.. The marker provides selection in rich medium containing 1 mM 4-chloro-dl-phenylalanine.An integrative vector based on this marker facilitates the construction of …
Sequential and counter-selectable cassettes for fission yeast
WebOct 7, 2024 · In E. coli, galK can be used as a positive selection marker on galactose as well as a counter-selectable marker by adding the toxic galactose analog 2-deoxy … Webampicillin selection. However, after the transformants were allowed to recover for several generations, their survival rates were similar on ampicillin plates and on tetracycline plates. The pBR322 plasmid is a commonly used cloning vector that contains both the ampicillin and tetracycline resistance genes as selectable markers. camerounvs
Synthetic counter-selection markers and their application …
WebMay 16, 2024 · Other counter-selection markers have been described and reviewed , but most of them were developed for use with E. coli and may have limited applicability outside of the Enterobacteriaceae. Choice of the counter-selection marker(s) depends largely on the physiology of the host bacterium. WebJun 9, 2024 · • The counter-selection marker benefits the gene targeting in S. elongatus UTEX2973. • Twentry-three small regulatory RNAs were fully deleted via the novel gene targeting method. Due to its robustness to environmental stresses and fast growth, Synechococcus elongatus UTEX2973 is developed as a new model for researches on … WebApr 3, 2024 · Abstract Escherichia coli phenylalanyl-tRNA synthetase, α-subunit (ePheS) can be useful as a counterselection marker since its A294G variant misincorporates 4 … cameroun vs ouzbékistan score