Phospho-acc
WebThe phosphorylation of ACC occurs downstream to AMPKα, and both Berberine and Orlistat effectively restored the levels of Phospho ACC (Ser-79) in the liver of AEBN-treated mice to control level... WebPhosphoric acids and phosphates. Pyrophosphoric acid. A phosphoric acid, in the general sense, is a phosphorus oxoacid in which each phosphorus (P) atom is in the oxidation …
Phospho-acc
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WebACC (Acetyl-CoA carboxylase) is an important regulator of fatty acid metabolism. By catalyzing the carboxylation of acetyl-CoA to malonyl-CoA, ACC serves as a novel biomarker or drug target in diabetes and obesity research. The enzymatic activity of ACC is inhibited by phosphorylation on Ser-79 residue. WebSep 13, 2024 · DSG treatment upregulated expression of lipolysis proteins, including phospho-AMP activated protein kinase (p-AMPK), phospho-acetyl-coA carboxylase (p-ACC) and carnitine acyl transferase 1A (CPT-1A), and inhibited expression of lipid synthesis-related proteins, including sterol regulatory element-binding protein 1c (SREBP-1c) and …
WebAug 1, 2006 · The phosphorylation of AMPK and its downstream target, ACC, was assessed by using immunoblots with phospho-Thr-172 AMPKα (pAMPK) and phospho-Ser-79 ACC1 (Ser221 ACC2; pACC) antibodies. The levels of phosphorylated AMPK and the sum of phosphorylated ACC1 and ACC2 were normalized to endogenous AMPKα levels and … WebAnti-phospho-acetyl-CoA carboxylase (ACC) (Ser-79) and antiphospho-CREB (Ser-133) were obtained from Upstate Biotechnology (Milton Keynes, United Kingdom). Anti-phospho-AMPK (Thr-172) and anti-β-actin antibodies were supplied by Cell Signaling (New England Biolabs) and Sigma-Aldrich (Saint Quentin Fallavier, France), respectively.
WebMar 21, 2024 · Furthermore, we also used a phospho-ACC (Ser79) cellular HTRF assay kit (96-well plate reader assay format), which we optimized for this cell line to measure activation of AMPK in a linear range across a number of doses of compounds, including A-769662 and an AMP mimetic prodrug, 5-aminoimidazole-4-carboxamide riboside (AICAR) … WebPhospho-ACC and phospho- gastrocnemius (GAST), and tibialis anterior (TA) muscles were AMPK antibodies (Cell Signaling Technology, Beverly, MA) were frozen between metal tongs at the temperature of liquid nitrogen and diluted 1:4,000 in 1% BSA dissolved in TBST.
Acetyl-CoA carboxylase (ACC) is a biotin-dependent enzyme (EC 6.4.1.2) that catalyzes the irreversible carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). ACC is a multi-subunit enzyme in most prokaryotes and in the chloroplasts of most plants and algae, whereas it is a large, multi-domai…
early textile study groupWebIncreased expression of phospho-acetyl-CoA carboxylase protein is an independent prognostic factor for human gastric cancer without lymph node metastasis. Upregulation … csulb education majorWebApr 4, 2010 · The primary antibodies used were as follows: PKM1/2 (antibody 3186), ACC (antibody 3676), phospho-ACC(Ser79) (antibody 3661), pan-AMPKα (antibody 2603), phospho-AMPKα(Thr172) (antibody 2535) were from Cell Signaling Technology. Hexokinase II (AB3279) and LDHA (AB1222) were from Millipore. csulb education minorWebApr 11, 2024 · All phospho-mutant mice had a C57BL/6 genetic background. All mice were bred in a specific-pathogen-free (SPF) environment. All animal experiments were approved by the Animal Ethics Committee of Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China (Nos. HKDL-2016-221 and SH9H-2024-A1-1). earlythWebLysates from F4 to F1 granulosa and theca cells were prepared and subjected to Western blotting using anti-phospho-AMPKα-Thr172, anti-AMPKα1/2 (α-pan AMPK antibodies … early text based computer gamesWebPhospho-Acetyl-CoA Carboxylase (Ser79) Antibody detects endogenous levels of ACC only when phosphorylated at serine 79. The antibody … early text to speechWebPhospho-ACC and phospho- gastrocnemius (GAST), and tibialis anterior (TA) muscles were AMPK antibodies (Cell Signaling Technology, Beverly, MA) were frozen between metal … early texas western town