Phospho antibody array

Author: aktp

August undefined, 2024

Webwith those identiﬁed in the phospho-antibody array analysis, we identiﬁed 38 protein kinases that are regulated by SII stimulation (Table S3). Included in this group of 38 are kinases in the MAPK Fig. 1. Human phospho-antibody array analysis reveals that phosphoryla-tion events induced by SII are primarily β-arrestin–dependent. (A) Normal- WebAug 24, 2005 · Anti-mTOR (phospho S2448) antibody See all mTOR primary antibodies Description Rabbit polyclonal to mTOR (phospho S2448) Host species Rabbit Tested applications Suitable for: IHC-P, WB more details …

Human and Mouse Phospho-PLCG2 (Tyr753) ELISA RayBiotech

WebNov 15, 2024 · This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-H2AX and total H2AX. An anti-pan H2AX antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and H2AX present in a sample is bound to the wells by the immobilized antibody. WebR&D Systems™ Human Phospho-Receptor Tyrosine Kinase (RTK) Array Kit (Catalog # ARY001B) is a screening tool designed to simultaneously detect the relative phosphorylation of 49 different RTKs. This kit eliminates the need for numerous immunoprecipitation (IP) and/or Western blot experiments. flewfie\u0027s adventure switch

Phospho Explorer Antibody Array - Full Moon BioSystems

WebThe antibody array membranes were washed and cocktail of biotinylated anti-EGFR was used to detect phosphorylated proteins on activated receptors. After incubation with HRP-Conjugated Streptavidin, the signals … WebDetection Antibody Cocktail B (blue cap) - One vial of lyophilized biotinylated antibodies for use with Part B membranes. Before use, reconstitute Detection Antibody Cocktail B in 100 μL of deionized or distilled water. 1X Array Buffer 2/3 - Add 2.0 mL of 5X Array Buffer 2 Concentrate to 8.0 mL of Array Buffer 3. Prepare fresh for each use. WebRayBio ® G-Series Human Receptor Tyrosine Kinase Phosphorylation Antibody Array 1 Kit. Detects 71 Human RTKs. Suitable for all liquid sample types but intended for use with cell and tissue lysates. $480.00 CODE: AAH-PRTK-G1-4 G-Series Size: Quantity: + − Add To Cart View all product options Description Citations (7) Reviews Ask a question Files chelsea apartments tyler tx

Proteome Profiler Mouse Phospho-RTK Array Kit

A reverse phase protein array based phospho-antibody …

WebAntibody Array Analysis CREB Pathway Phosphorylation Antibody Array The cAMP-response element binding protein (CREB) is a member of the bZIP transcription factor … WebAntibody Array Kit for studying IGF1R beta in the research area. ... between lysate protein concentration from untreated and IGF-I treated MCF-7 cells and the relative fluorescence of phospho-IGF-IR (panTyr), phospho-Akt (Ser473) and phospho-p44/42 (Thr202/Tyr204) is shown. MCF-7 cells were starved for 24 hours, then treated with 100 ng/ml IGF ... chelsea apothecary nycWeb46 rows · Each Human Phospho-Kinase Antibody Array Kit contains most of the necessary components for a ready-to-run assay without the need for specialized equipment. 8 … flew flown grew grown meme

"WebProtocol Outline. Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 µl of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared 1X HRP-Streptavidin to each well. " - Phospho antibody array

Phospho antibody array

WebThe antibody array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a … WebApoptosis Phospho Antibody Array features 247 highly specific antibodies related to apoptosis and cell death. The array is designed for protein phosphorylation profiling and …

Did you know?

WebThe Proteome Profiler Human Phospho-RTK Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). WebThe array is designed for protein phosphorylation profiling in cell and tissue samples from human, mouse, and rat. Each package contains two identical array slides for analyzing …

WebHuman Phosphorylation Pathway Profiling Array C55 Rating: 1 Review 2 Citations Detects 55 phosphorylated human proteins in 5 signaling pathways: MAPK, AKT, JAK/STAT, NFκB, and TGFβ. Suitable for all liquid sample types but intended for use with cell and tissue lysates. MANUAL / SDS $901.00 Size In stock SKU AAH-PPP-1-2 + Add to Cart WebMay 11, 2024 · An antibody specific to the phosphorylation-of-interest is added to the membrane. The phosphorylation antibody is then bound by a secondary antibody conjugated to horseradish peroxidase (HRP). Antibody binding is detected when an HRP substrate is added, resulting in chemiluminescence.

WebRabbit polyclonal antibody raised against synthetic phosphopeptide of ABL1/ABL2. Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y393/Y429 of human ABL1/ABL2. (PAB12190) - Products - Abnova

WebSep 20, 2024 · The phospho-protein arrays used in our experiments are based on analysis of tissue samples on nitrocellulose membranes, where specific antibodies against selected …

WebThe Proteome Profiler Mouse Phospho-RTK Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). chelsea apothecaryWebPhospho-specific antibodies are used to assess phosphorylation status using fluorometric (Figure 4) or colorimetric detection systems. Furthermore, the phospho-protein and total protein are simultaneously detected in the same microplate well. flewfie\u0027s adventure reviewWebPhospho-specific Antibody Arrays Referenced in Primary Literature. There are over 200 references to phospho-specific, membrane-based antibody arrays in the primary literature. Proteome Profiler™ Phospho-specific Antibody Arrays offered by R&D Systems account for 91% of those referenced. chelsea app launcherWebFeb 28, 2024 · Building antibody arrays using capture reagents targeting proteins in the signalling cascade of interest has enabled researchers to investigate changes in protein profiles and modifications in signalling pathways in … flew for free crosswordWebCancer/Apoptosis Phospho Antibody Array features 155 highly specific antibodies related to apoptosis pathways in cancer. The array is designed for protein phosphorylation profiling … flew high crosswordWebNeuroscience Phospho Antibody Array Ref: PNS170 Sizes: 2 Slides/PK From: €996.00 Product detail Human Cytokine Array Q440 Ref: QAH-CAA-440-1 Sizes: 8 Sample Kit From: €4,701.00 Product detail Human Cytokine Array Q440 Ref: QAH-CAA-440-2 Sizes: 22 Sample Kit From: €8,462.00 Product detail Human Cytokine Array Q440 Ref: QAH-CAA-440-4 flew flowWebOct 16, 2024 · The Proteome Profiler Mouse Phospho-RTK Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). flew great