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Psichecktm-2 vector

WebpsiCHECK™-2 Vector Part Numbers: C8021 Vector for Optimizing RNA Interference Assays Monitor changes in expression of a target gene fused to a reporter gene; decreases in luciferase activity correlate with RNAi … WebAug 18, 2024 · The 3′-UTR sequence of PPARγ was cloned and inserted into the PsicheckTM-2 vector to generate PsicheckTM-2-PPARγ-WT. The miR-377 binding site in …

psiCHECK™-2 Vector RNAi Assay - Promega

WebHere we demonstrate the use of the psiCHECK™-2 Vector as a biosensor for miRNA-21 (miR-21) in different cell lines and as a tool to test an miRNA target. Generating an miRNA … WebFrom the psiCHECK-2 vector (Promega, Cat. No. C8021), a 231-nucleotide (nt) region was selected from the firefly luciferase gene sequence available for (5) Tumor elimination by … roissy international https://bethesdaautoservices.com

Biomedicines Free Full-Text Hypoxia-Induced Downregulation of …

Webthe PsicheckTM-2 vector to generate PsicheckTM-2-PPAR γ-WT.ThemiR-377bindingsiteinPPAR wasthenmutated,andthemutantfragmentwas 2-PPARγ-MT.Forty … WebMyocyte enhancer factor (MEF2) is a family of transcrip- tion factors that binds to an A/T-rich DNA element in the promoter regions of muscle-specificgenes(12). MEF2 exerts a diversity of function during myogenic differentiation and is involved in the control of various aspects of muscle fiber properties (13). WebJun 17, 2024 · Example 2: Convert Matrix to Vector (sorted by rows) Using c() function. The following code shows how to convert a matrix to a vector (sorted by rows) using the c() function: #convert matrix to vector (sorted by rows) new_vector <- c(t(my_matrix)) #display vector new_vector [1] 1 6 11 16 2 7 12 17 3 8 13 18 4 9 14 19 5 10 15 20 Example 3 ... roissybus terminal 2e

MicroRNA-129-5p inhibits C2C12 myogenesis and represses …

Category:psiCHECK-2 Vector, 20 µg, Promega VWR

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Psichecktm-2 vector

LncRNA XIST/miR-200c regulates the stemness properties and ...

WebYou have 2 remaining attempts. You have 1 remaining attempt. Contact Customer Service. Forgot Password? Close Account Locked. To protect your privacy, your account has been … WebAug 13, 2024 · The 3′UTR of CREBL2 and PCBP2 were sub-cloned into the psiCHECK-2 vector (Promega, USA) downstream of the Renilla luciferase reporter gene. Flanking sequence on each side was synthetized with a short extension containing cleavage sites for XhoI (CTCGAG) and NotI (GCGGCCGC), and the primer sequences are shown in Table …

Psichecktm-2 vector

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WebpsiCHECK™ -1 和psiCHECK™ -2 载体用于对RNA干扰(RNAi)的早期优化提供快速的定量方法。此载体可监测与报告基因融合的目的基因表达的变化。两种载体都应用海肾萤光素酶作 … WebApr 9, 2024 · 作者签名：产m堤日期：2口出年‘月侈日学位论文版权使用授权书学位论文作者完全了解华中师范大学有关保留、使用学位论文的规定，即：研究生在校攻读学位期间论文工作的知识产权单位属华中师范大学。 ... －CHECK2 recombinant expressive vector carrying ...

Webinserted into psiCHECKTM-2 Vector (Promega, Madison, WI, USA) to construct plasmids used in the following dual-luciferase reporter assays. The open reading frame (ORF) of HMGB1 was cloned and inserted into pCMV6AC-GFP (Origene, - Rockville, MD, USA) vector to generate HMGB1. The primer sequences were listed in Supplementary Table 5. WebpsiCHECKTM-2 dual-luciferase reporter plasmid (Promega, cat. no. E1910) to produce psiCHECK-WT-NLRP3 and the mutant vector psiCHECK-MT-NLRP3. Then, the two plasmids were co-transfected into HUVECs with either miR-22 mimic, inhibitor or miR-22 control. For the reporter assays, HUVECs were harvested and lysed and then

WebFeb 8, 2024 · PsiCHECKTM‐2 vector without inserted gene was used as negative control (NC) plasmid. The inserted sequences were listed in Table. Table.1. 1. Following the procedures provided by Ribobio and Promega, A7r5 cells were co‐transfected with PsiCHECKTM‐2 vector (WT/MUT/NC) and oligonucleotides (miR‐137 mimic or mimic … WebBy cloning the 3’UTRs of collagen IV subunits into the psiCHECKTM-2 vector, we found that seven of the eight sites in the Col4a3–Col4a6 gene complementary to miR-29 were significantly repressed...

Web2.2 测序及序列对比分析 含有hsa-miR-181b结合位点及缺失hsa-miR-181b结合位点的荧光素酶报告载体psiCHECK2-TNF和psiCHECK2-TNF-mut测序结果(图3、图4)。 可见TNF的目标序列和突变序列均成功地克隆入双萤光报告载体psiCHECK2，可用于后续实验。

WebThen, 100 ng psiCHECK‑2 luciferase reporter plasmids containing WT and M uT 3'-uTr of Bax were co-transfected into B nlcl 2 cells with mi r-214 mimic (100 nM) or mimic control (100 nM) for 48 h using ipofectaminel®2000 (Invitrogen; Thermo Fisher Scientific, Inc.). outback del amo mallWebApr 15, 2024 · Fig. 2: Néel vector control by cooling in opposite magnetic fields. a Optical image and its MCD image at 280 K of the front ab plane in a 500 μm-thick crystal. outback delivery athens gaWebPromega psichecktm 2 vector Psichecktm 2 Vector, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, … outback del rey telefoneWebpsiCHECKTM-2 dual-luciferase reporter plasmid (Promega, cat. no. E1910) to produce psiCHECK-WT-NLRP3 and the mutant vector psiCHECK-MT-NLRP3. Then, the two … outback del rey bhWebJan 15, 2024 · The mutant MAP3K1 sequence was also attached to the psiCHECKTM-2 vector by change the binding sites from TCTTGCCA to AGAACGGT. The wild-type or mutant RASA1 vector was constructed as described above. The ... Inhibitor-NC and goat miR-31-5p Inhibitor were co-transfected with psiCHECKTM-2 goat MAP3K1-3′UTR (wild-type … roissy cergy busWeb鸡IGFBP2基因3′UTR区1196C_A单核苷酸多态性的功能性鉴定及分析.pdf roissy infoWebエムエスアイコンピュータージャパンは4月13日、プロeスポーツプレイヤー向けをうたったハイエンド構成の16型ゲーミングノートPC「Vector GP68HX 12V ... roi thaumaturge def